In the rapidly evolving field of molecular biology, having reliable controls is essential for validating experimental methods. Tsingke offers a comprehensive selection of custom RNA synthesis solutions, including effective siRNA positive controls and non-specific negative controls, ensuring researchers can confidently assess the feasibility and specificity of their experimental designs.
Effective siRNA Positive Controls
Positive controls play a vital role in siRNA experiments, as they verify the effectiveness of the experimental methods. Tsingke provides reliable siRNA positive controls targeting key housekeeping genes such as GAPDH and ACTB, along with reporter genes like GFP and EGFP. By utilizing these verified siRNAs, researchers can establish a benchmark for their experiments, ensuring that the methods employed are robust and dependable. This focus on quality is part of Tsingke’s commitment to excellence in custom RNA synthesis.
Non-Specific siRNA Negative Controls
In addition to positive controls, Tsingke offers negative controls, which are non-specific siRNAs designed to demonstrate the specificity of siRNA effects. These controls can be selected from universal or scrambled sequences tailored to specific experimental requirements. By incorporating these negative controls, researchers can discern genuine siRNA effects from background noise, enhancing the accuracy of their results. This careful consideration of control options reflects Tsingke’s dedication to providing comprehensive custom RNA synthesis services.
Fluorescent Labeling for Enhanced Detection
To facilitate effective monitoring, Tsingke provides siRNAs labeled with various fluorescent dyes as part of its custom RNA synthesis offerings. The FAM dye has maximum absorption at 495 nm and emits at 520 nm, while Cy3 has a maximum absorption at 550 nm and emits at 565 nm. For deeper tissue penetration and sensitivity, Cy5 is also available, with a maximum absorption at 643 nm and emission at 667 nm. These fluorescent labels enable researchers to visualize and quantify siRNA activity in real time, significantly enhancing experimental analysis.
Conclusion
In conclusion, Tsingke stands out as a reliable provider of custom RNA synthesis solutions, catering to the diverse needs of researchers in molecular biology. With effective siRNA positive controls targeting essential genes, non-specific negative controls for specificity validation, and advanced fluorescent labeling options, Tsingke supports scientists in achieving accurate and reliable results in their experiments. Trust Tsingke for your custom RNA synthesis needs and elevate the quality of your research today.
